Effect of Various Biotic and Abiotic Factors on the Activity of Tannase

Extracellular tannase production by Trichophyton rubrum has been evaluated using submerged fermentation (SmF) at different abiotic factors like pH, temperatures and biotic factors like carbon, nitrogen and concentration of gallic acid. T. rubrum produced the highest tannase level at a temperature of 30°C where the enzyme production was 23.52 U/mL and pH of 5.5 where enzyme production was 32.62 U/mL) on 3 of rd inoculation. Among the different carbon and nitrogen sources tested, sucrose and casein hydrolysate respectively were found to the best in terms of the maximal production of tannase. Further, the enzyme production was coincided with the growth pattern of the fungus. The study may be more useful to optimize the media for the maximum production of tannase in which gallic acid as the substrate.